Fig. 1. Lic is required for depletion of scrib-induced invasive cell migration in wing discs.

Fluorescent micrographs of third instar wing discs (a–p) are shown. The dotted line depicts the area where cell migration occurs with ptc > GFP in the imaginal wing disc (a). ptc-Gal4 driven overexpression of scrib-RNAi promoted cells from A/P boundary migrating to posterior and MMP1 activation (e–g), compared to the GFP-expressing control (b–d). As a positive control, Puc blocked the cell migration phenotype and MMP1 staining (h–j). Expression of two independent lic-RNAi lines significantly inhibited cell invasion and MMP1 expression (k–p). Scale bars, 20 μm. The number of migrated cells were quantified and shown in (q), and one-way ANOVA test was used to calculate statistical significance, n ≥ 17, mean + s.d., ***P < 0.001