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. 2019 Feb 15;10(3):149. doi: 10.1038/s41419-019-1350-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Fluorescent (a–e, g–k, m–q) and light (f, l, r) micrographs of third instar wing discs are shown. Compared with the control (a–f), ectopic expression of MKK3 induced cell migration (g) accompanied by activation of MMP1 (h), integrin (i), TRE-RFP (j), p-JNK (k) and puc-LacZ (l), which were significantly suppressed by co-expression of Puc (m–r). Scale bars, 20 μm. Statistics of migrated cell numbers was shown in (s), and One-way ANOVA test was used to calculate statistical significance, n ≥ 14, mean + s.d., ***P < 0.001