Fig. 4. Downregulation of KLF5 inhibits the LPA-induced proliferation of SV40 MES13 cells.
a Cells were transfected with a scrambled siRNA (siCon) or KLF5 siRNA (siKLF5) for 48 h and treated with LPA (10 μM) for 6 h, and the levels of the KLF5 and p27Kip1 proteins were determined using western blotting. b, c The relative levels of the proteins were normalized to β-actin and quantified using ImageJ software (n = 3 independent experiments). d Cell morphology was examined using light microscopy (original magnification, × 100), and (e) cell proliferation was analyzed using CCK-8 assay after treatment with 10 μM LPA and transfection of the KLF5 siRNA or scrambled siRNA for 24 h (n = 3 independent experiments). *p < 0.05, **p < 0.01. Data are presented as means ± SEM