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. 2019 Feb 15;51(2):18. doi: 10.1038/s12276-019-0217-3

Fig. 5. LPA increases MAPK activation and Egr1 expression in SV40 MES13 cells.

Fig. 5

a Western blots showing levels of MAPK proteins (p-Erk, total Erk, p-JNK, total JNK, p-p38, and total p38) in cells treated with 10 μM LPA for 5 or 15 min. b The relative protein levels were normalized to β-actin and quantified using ImageJ software (n = 3 independent experiments). c The expression of the Egr1 mRNA was analyzed using qRT-PCR in cells treated with 10 μM LPA for 5 or 15 min (n = 3 independent experiments). d Western blots showing the levels of the Egr1 and β-actin proteins in cells treated with 10 μM LPA for 6 h. e The relative Egr1 levels were normalized to β-actin and quantified using ImageJ software (n = 3 independent experiments). f Western blot showing the levels of the Egr1 protein in the renal cortex of 9-week-old wild-type (WT) and db/db mice. g The results were quantified, and β-actin was used as a loading control (n = 3–4). *p < 0.05, **p < 0.01. Data are presented as means ± SEM