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. 2019 Feb 15;10(3):156. doi: 10.1038/s41419-019-1405-8

Fig. 3. PLOD3 knockdown induces caspase-dependent apoptosis in R-H460 cells.

Fig. 3

a, b Cell death in cells transfected with 40 nM siPLOD3 as determined by AV/PI staining and western blotting at 12-h intervals. *P < 0.05. c R-H460 cells were treated with or without 40 µM Z-VAD-FMK (a pan-caspase inhibitor) after transfection with PLOD3 siRNA. Cell death was measured 48 h after treatment by AV/PI staining and western blotting. **P < 0.01. d Analysis of caspase activity in R-H460 cells 48 h after transfection with PLOD3 siRNA by ELISA. Data were collected using a Multiskan EX at 405 nm. *P < 0.05; **P < 0.01. e R-H460 cells were treated with Z-VAD-FMK (40 µM), Z-VDVAD-FMK (40 µM), Z-DEVD-FMK (40 µM), or Z-YVAD-FMK (40 µM) and then subjected to AV/PI staining and western blotting for active-caspase-3. **P < 0.01; ***P < 0.001