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. 2019 Feb 15;10(3):146. doi: 10.1038/s41419-019-1323-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Cell viability was measured with MTT assay. a Cell viability of melanoma cells treated with various concentrations of the free αM and the αM/MPEG-PCL nanomicelles for 24 h, 48 h, and 72 h. Values represent mean ± SD (n = 3, in triplicate, *P < 0.05; **P < 0.01; ***P < 0.001 compared to control). b The effects of the free αM and the αM/MPEG-PCL nanomicelles on colony formation in melanoma cells and the statistic assay of colony formation experiments. Data are expressed as mean ± SD (from three independent experiments), *P < 0.05; **P < 0.01; ***P < 0.001 compared to vehicle (0 μM group). c Non-tumor cell lines (LO2, Vero, and HEK293T) were incubated with a series of concentrations of the αM/MPEG-PCL nanomicelles for 48 h. Values represent mean ± SD (n = 3, in triplicate, *P < 0.05; **P < 0.01; ***P < 0.001 compared to control)