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. 2019 Feb 15;10:771. doi: 10.1038/s41467-019-08686-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A fuzzy charge–charge interaction enhances PP1α C-tail binding to ASPP2. a Fully annotated 2D [¹H,¹⁵N] HSQC of PP1_301-330. b 2D [¹H,¹⁵N] HSQC of PP1^301-330 (black) in presence of a 2 (violet) or 4 (green) molar excess of ASPP2. c Comparative analysis of ¹⁵N[¹H] NOE of free PP1α C-tail (black bars) and PP1α C-tail: ASPP2 (grey bars); missing peaks are due to overlap, making quantitative intensity analysis impossible. Error bars represent standard deviations. d Comparative analysis of ¹⁵N transverse relaxation (R₂) data of free PP1α C-tail (black bars) and PP1α C-tail: ASPP2 (grey bars). Error bars represent standard deviations