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. 2019 Jan 15;10(2):34. doi: 10.1038/s41419-018-1169-6

Fig. 4. Suppression of Sirt3 expression in nephrolithiasis in vitro was dependent on activation of the MAPK pathway.

Fig. 4

a SB203580 (P38 inhibitor), PD98059 (JNK inhibitor) and SP6000125 (ERK inhibitor) significantly increased Sirt3 expression, while PDTC (NF-κB inhibitor), SRP1/2 (mTOR inhibitor), Ly294002 (PI3K inhibitor), rapamycin (mTOR inhibitor), AZD1480 (JakStat inhibitor) and GO6976 (PKCα/PKCβ1 inhibitor) decreased Sirt3 expression. b These inhibitors were used to determine the pathways responsible for apoptosis and necrosis under CaOx stimulation. c SB203580 (P38 inhibitor), PD98059 (JNK inhibitor) and SP6000125 (ERK inhibitor) significantly reduced apoptosis and necrosis. d The phosphorylation levels of ERK, JNK and P38 were increased in a time-dependent manner under CaOx stimulation. e TEC apoptosis and necrosis were increased with sirt3 knockdown. Data are shown as the means ± S.D.; n = 3 per group