Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 15;10(2):34. doi: 10.1038/s41419-018-1169-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Immunoprecipitation assays demonstrated that Sirt3 overexpression via resveratrol treatment decreased acetylated FoxO3a expression in the kidney in vivo. b Similarly, overexpression of Sirt3 in TEC in vitro significantly decreased the FoxO3a acetylation level under CaOx stimulation. c Immunofluorescence assays indicated that Sirt3 interacted with FoxO3a most abundantly around the nucleus (DAPI: blue, FoxO3a: green and Sirt3: red). Scale bar: 60 μm. d Co-immunoprecipitation (co-IP) assays confirmed the interaction between Sirt3 and FoxO3a. e Overexpression of a catalytic mutant of Sirt3 (Sirt3^H248Y) did not influence FoxO3a acetylation. f Overexpression of Sirt3^H248Y did not reduce ROS activity in TEC. g Overexpression of Sirt3^H248Y did not decrease TEC necrosis under CaOx stimulation. h Overexpression of Sirt3^H248Y increased cleaved caspase-3 and Bax expression and reduced Bcl-2 expression. Data are shown as the means ± S.D.; n = 6 mice per group for an in vivo experiment. n = 3 per group for an in vitro experiment