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. 2019 Feb 15;24:14. doi: 10.1186/s11658-019-0140-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Primer specificity and average raw cycle thresholds. a – The amplification specificity of all candidate reference gene primer sets. cDNA was isolated from undifferentiated human ASCs. Lanes 1–10: GAPDH, TBP, EF1A, LMNA, RPS18, PSMD5, MRPL19, TCRF, CCNA2 and GUSB. B and C – Raw quantitative PCR C_T values for candidate reference genes during proliferation (b) and adipogenesis (c). Each gene was amplified in 15 (proliferation) or 24 (adipogenesis) different biological samples in duplicates. Values are presented as means +/− SEM