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. 2019 Feb 15;20:136. doi: 10.1186/s12864-019-5519-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Comparison of the expressing profiles for selected DEGs between RT-qPCR analysis and RNA-Seq results. To confirm the reliability of Illumina sequencing technology, 18 candidate genes were randomly selected and their expressions were confirmed by RT-qPCR using RT-primers. Differential expression was observed for all the candidate genes, indicating that they are involved in the regulatory networks that are active during low-K stress condition. The results of RT-qPCR showed that the RT-qPCR assessments of these 18 genes were consistent with those of sequencing analysis