Fig. 4.

Evaluation of antibacterial activity of 29-aa-abaecin with or without cecropin B. a The antibacterial activities of 29-aa abaecin with or without cecropin B against B. subtilis. Agar plates spread with 100 μl of B. subtilis liquid culture grown overnight were punctured and dropped with 10 μl of abaecin or cecropin B or both peptides, which were purified and resuspended in 1X PBS, then incubated at 37 °C for 16 h. The antimicrobial peptides and their amount treated into the corresponding wells are represented in (b). b Evaluation of inhibitory effect by antimicrobial peptides on B. subtilis by measuring the inhibited zone area. The inhibited zone areas of B. subtilis were extrapolated using ImageJ software. Scale bars represent 1 cm