Fig. 6.

Combination of JQ1 with EGFR inhibitor reduces JQ1 resistant HCC tumor growth. a 97-H cells (5 × 10⁶) were injected into the flanks of CB17/SCID mice. After the subcutaneous tumors reached a size of 10 cm³, mice were treated with vehicle, JQ1 (50 mg/kg), ERL (50 mg/kg) or a combination of the drugs every 2 days. Tumor volumes were monitored during the treatments. Pictures of tumor removed are shown at the right. b Tumor weights are shown after 3 weeks of treatment. c Western blot analysis of MYC and p-MYC-Ser62 in tumor lysates. GAPDH was used as a loading control. d Representative immunohistochemistry images. Tumors from mice treated with vehicle, JQ1, ERL or the combination were stained with MYC, p-MYC-Ser62 antibodies and TUNEL. Data are presented as mean ± s.d. **p < 0.01, Scale bars, 20 μm