Fig. 4.

Effect of the metabolic/bioenergetic state of the mitochondria on the conductance change. (a) Addition of different reagents to the reservoir during the measurement alters the membrane potential of mitochondria. Initially mitochondria were suspended in a substrate free buffer and flown into the channel; next mitochondria were energized by adding sodium succinate to a final concentration of 10 mM; finally CCCP was added to the reservoir (final concentration 50 µM) to depolarize the mitochondria; (b) Normalized scatter plot showing the distribution of the events observed for energized and de-energized mitochondria. Detection of energized mitochondria has been reproduced nine times on nine separately fabricated devices.