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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Arch Biochem Biophys. 2018 Dec 20;663:22–33. doi: 10.1016/j.abb.2018.12.017

Fig. 1.

Fig. 1.

IF and EM analysis of EDL fibers from aged and aged-trained mice. A and B) Representative IF images showing RyR1 and TOM20 double-staining (marking the position of CRUs and mitochondria, respectively). C-F) Representative EM images showing the position of mitochondria (pointed by small arrows in C and D) and the organization of TTs (pointed by arrowheads in E and F). In C, the empty arrow point to an area with abnormal mitochondria distribution. G-L) Quantitative analysis of mitochondrial n./area, volume, disposition, and association with CRUs. Data are shown as mean ± SEM; n = number of EDL fibers analyzed; *p < 0.05 as evaluated by two-tailed unpaired Student’s t-test. Scale bars: A and B, 5 μm (insets 2 μm); C–F, 2 μm.