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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Arch Biochem Biophys. 2018 Dec 20;663:22–33. doi: 10.1016/j.abb.2018.12.017

Fig. 7.

Fig. 7.

IF and EM analysis of EDL fibers from control, denervated, and reinnervated rats. A, C and E) Representative IF images showing fibers double immuno-labeled with RyR1 and TOM20 antibodies marking the position of CRUs and mitochondria, respectively. B, D and F) Representative EM images showing the disposition of mitochondria in EDL fibers from control, denervated, and reinnervated rats. In B and F black arrows point to mitochondria properly positioned at the I band, while in D empty arrows point to mitochondria forming longitudinal columns between myofibrils. G-L) Quantitative analysis of mitochondrial n./area, volume, disposition, and association with CRUs. Data are shown as mean ± SEM; n = number of fibers analyzed; *p < 0.05 as evaluated by one-way ANOVA followed by post-hoc Tuckey test. Scale bars: A, C and E, 5 μm (insets 2 μm); B, D and F, 1 μm.