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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: Cancer Res. 2018 Dec 18;79(4):760–772. doi: 10.1158/0008-5472.CAN-18-2297

Figure 2.

Figure 2.

Loss of H2bub1 is an early event in serous tumorigenesis. (A) Immunohistochemical analysis of H2Bub1 expression in morphologically normal fallopian tube epithelium (FTE), serous tubal intraepithelial carcinoma (STIC), and invasive high-grade serous cancer (HGSOC), with p53 staining marking the carcinoma cells. Abundant H2Bub1 is expressed in both secretory cells (between arrowheads) and ciliated cells of morphologically normal FTE, but it is strongly downregulated or lost in STIC and invasive HGSOC. All micrographs (40x, Scale bar = 20 μm) were imaged from one representative case. (B) Low magnification (10x) images of (A) displaying the lesions (arrows) in continuity with normal FTE. (C) Composite H1Bub1 immunostaining scores (range - 0–12), obtained by multiplying semiquantitative scores for stain intensity and distribution (each scored as: 0+, 1+, 2+, 3+;) (horizontal bars – median; P - Mann-Whitney test).