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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: Cancer Res. 2018 Dec 27;79(4):807–818. doi: 10.1158/0008-5472.CAN-18-2209

Figure 1.

Figure 1.

Pep8 modulates the Rbm38-eIF4E complex. A Visual representation of the peptides used in this study. B Immunoblot for the competitive pull-down assay for GST-Rbm38 and His-eIF4E (2 μg) in the presence of peptide modulators (375 nM). Standard deviation of relative eIF4E band intensities was 0.1 (Pep23) and 0.08 (Pep8). C Visual representation of the docking of Pep8 to eIF4E based on REMDS. D Visual depiction of the key residues involved in the Pep8-eIF4E interaction. E Immunoblot for the pull-down of eIF4E with TentaGel-bound peptides. F Immunoblot for the competitive pull-down assay for GST-Rbm38 and eIF4E (5 μM) with the addition of Pep8 derivatives (5 μM). Standard deviation of relative eIF4E band intensities was 0.07 (Pep8), 0.003 (Pep8S-D) and 0.07 (Pep8S-K). All western blot figures are representative data of at least 2 independent replicates.