Fig. 2.

(a) 3D reconstruction of a D-FFOCT image stack in explanted macaque retina over a 120 by 120 μm field of view. Note that FFOCT signal is damped with increasing penetration depth, so that upper retinal layers are more clearly visible than lower ones. (b, c, e) En-face images of the (b) inner nuclear layer, (c) outer nuclear layer and (e) photoreceptor layer presenting a similar appearance to two-photon fluorescence imaging [32] and (d) reconstructed cross-section at the location represented by the red dotted line in (a). The cross-section in (d) was linearly interpolated to obtain a unitary pixel size ratio. (f) D-FFOCT image of a porcine retinal pigment epithelium cell culture [31]. (g) Overlay of colored D-FFOCT and FFOCT at the interface between the layers of the nerve fibers (white arrows point to nerve bundles that are very bright in static and invisible in dynamic mode), ganglion cells (blue and green cells, visible in dynamic mode) and inner plexiform (fibrous network, bottom left, visible in static mode). Samples were maintained in vitro in culture medium at room temperature during imaging.