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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Chem Neuroanat. 2018 Oct 23;96:1–6. doi: 10.1016/j.jchemneu.2018.10.006

Figure 3. Immunohistochemical analysis of cocaine exposure on hippocampal CCTβ expression.

Figure 3.

(A) IHC was performed on 10-μm sagittal sections to assess CCTβ protein expression in the hippocampus of saline- and cocaine-treated rats (magnification: X20). (B) Quantification and image analysis was performed using the ImageJ-based software package FIJI to obtain the relative optical density of DAB staining values. Relative optical density (O.D.) was calculated based on the maximum density of DAB staining values, which was derived based on the maximum intensity of an 8-bit image. Scale bar, 50 μm. Data indicate results from at least 3 separate animals (n=3) per group and are expressed as mean values ± SEM.