Figure 2.

Silencing of Kdm7a in ST2 inhibited adipocyte formation and promoted osteoblast differentiation. qRT‐PCR verified the silencing of Kdm7a in ST2 (A). Kdm7a silencing reduced adipocyte formation after adipogenic treatment (B). Oil red O extracted with isopropanol was measured at OD520 (C). The mRNA levels of PPARγ, C/EBPα, aP2 and adipsin (D) and protein levels of PPARγ, C/EBPα and aP2 (E) were down‐regulated in Kdm7a silencing cells. Alkaline phosphatase staining was enhanced in Kdm7a silencing cells after osteogenic treatment (F). The mRNA levels of Runx2, Osterix, Alp, Osteopontin and Osteocalcin (G) and protein levels of Runx2, Osterix, ALP and Osteopontin (H) were increased in Kdm7a silencing cells. Image scale in (B): 200 μm. Values are means ± SD (n = 3). *P < 0.05 vs control siRNA