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. 2019 Jan 13;23(3):1698–1713. doi: 10.1111/jcmm.14065

Figure 2.

Figure 2

Effects of TIPE2 on the proliferation and viability of human rectal adenocarcinoma cells. A, Fluorescence microscopy of TIPE2 in HR8348 and SW837 cells; original magnification 100×. B, The expression level of TIPE2 mRNA was examined by RT‐PCR. C, The protein expression of TIPE2 was examined by Western blotting. GAPDH was used as the loading control. D, The densitometry analysis of TIPE2 was performed, normalized to the corresponding GAPDH level. E, DNA replication activities of HR8348 and SW837 cells in each group were examined by EdU assay; original magnification 100×. F, The proliferation rate of each group was analysed. G, The percentages of viable cells were determined using MTS assay and the cell viability of the control group was taken as 100%. H, The clonogenic capacity was determined in HR8348 and SW837 cells. I, The numbers of colonies were calculated. Data are presented as mean ± SEM of three independent experiments; *P < 0.05, **P < 0.01 compared with the Mock group; # P < 0.05, ## P < 0.01 compared with the sh‐Scb group.