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. 2019 Jan 13;23(3):1698–1713. doi: 10.1111/jcmm.14065

Figure 3.

Figure 3

Effects of TIPE2 on the migration and invasion of human rectal adenocarcinoma cells. A, The effect of TIPE2 on cell migration was measured by wound healing assay; original magnification 100×. B, The migration rates of HR8348 and SW837 cells were calculated by the formula shown above. C, Soft agar assay was performed to examine the anchorage‐independent survival of cells; original magnification 100×. D, The number of colonies was calculated. E, Transwell assay was performed to assess the migration of HR8348 and SW837 cells; original magnification 200×. F, Transwell assay was performed to assess the invasion of HR8348 and SW837 cells; original magnification 200×. G, The numbers of the migrated cells were calculated. H, The numbers of the invasive cells were calculated. Data are presented as mean ± SEM of three independent experiments; *P < 0.05, **P < 0.01 compared with the Mock group; # P < 0.05, ## P < 0.01 compared with the sh‐Scb group.