Figure 3.

Ursolic acid decreases apoptosis in the heart of mice treated with doxorubicin. A, TUNEL staining of heart samples. a, Sham group; b, Doxorubicin + Ursolic acid group; c, Doxorubicin group (Bar = 20 μm). B, Ursolic acid significantly decreased cardiac cell apoptosis in mice treated with doxorubicin compared with the doxorubicin control (n = 5, *P < 0.01). Apoptotic cell nuclei were stained brown (arrow). C, Western blot detection of cleaved caspase‐3 in the mouse heart after treatment with doxorubicin. Lanes 1 to 3 represent the Sham, Doxorubicin and Doxorubicin + Ursolic acid treatment groups respectively. E. Ursolic acid significantly decreased cleaved caspase‐3 levels in the hearts of mice compared with doxorubicin (n = 5, *P < 0.01)