Figure 1.

Knockout of Cosmc induced Tn expression in CRC cell lines. A, A chart illustrated the biosynthetic pathway of mucin type O‐glycans and the significant role of Cosmc chaperone in the process of correct O‐glycosylation. B, The knockout of Cosmc chaperone and the degradation of T‐synthase were confirmed by the Western blotting. C, Flow cytometry analysis for the expression of Tn antigen in Cosmc deficiency cells. The percent of Tn positive cells in HCT116 and SW480 cells was 94.4% and 97.9% respectively