Figure 2.

Expression of Tn antigen enhanced the invasive properties. A and B, The prominently increased cell migration and invasion because of the expression of Tn antigen were analysed by transwell assays in both HCT116 and SW480 cells. The indicated migrated and invaded cells were quantified in five randomly chosen fields and presented as mean ± SD (**P < 0.01). C and D, Photographs of liver metastases and lung metastases formation in intrasplenic injection mouse models. E, The formation of liver metastases and lung metastases was confirmed by H&E staining. F, Photographs of metastatic lymph nodes in the mice of orthotopic implantation models. The formation of lymph node metastasis was confirmed by H&E staining. G, Survival rate of the mice in orthotopic implantation models was calculated. The overall survival time in Tn‐positive group was significantly reduced compared with the control (*P < 0.05)