Figure 3.

Expression of Tn antigen in human colorectal cancer promoted EMT. A, The expression of E‐cadherin, N‐cadherin, Vimentin and Snail in HCT116 and SW480 cells were analysed by western blotting. B and C, Relative mRNA expression of E‐cadherin, N‐cadherin, Snail, Slug and Vimentin were calculated by qRT‐PCR in HCT116 and SW480 cells. (*P < 0.05 and **P < 0.01). D, Immunohistochemistry staining in the xenografts in orthotopic implantation murine models showed that there was a reduced E‐cadherin expression but an increased Snail expression in Tn‐positive group