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. 2019 Jan 13;23(3):2083–2092. doi: 10.1111/jcmm.14117

Figure 4.

Figure 4

Increased H‐Ras expression contributed to the activated EMT in Tn‐positive cells. A and B, The significantly increased H‐Ras expression in Tn‐positive CRC cells and Tn‐ positive xenografts was confirmed by Real‐time PCR and Western blotting (*P < 0.05 and **P < 0.01). C and D, The ability of cell migration and invasion in Tn positive cells with H‐Ras knockdown and the control was analysed by transwell assays. Significantly reduced cell migration and invasion was detected after down‐regulation of H‐Ras in both HCT116Tn+ and SW480Tn+ cells (**P < 0.01). E and G, The influence of H‐Ras down‐regulation on the levels of E‐cadherin, N‐cadherin, Vimentin and Snail in both Tn‐positive cells was analysed by Western blot and Real‐time PCR (*P < 0.05)