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. 2019 Jan 13;23(3):2083–2092. doi: 10.1111/jcmm.14117

Figure 5.

Figure 5

Re‐expression of Cosmc in Tn positive cells down‐regulated the expression of H‐Ras. A, Re‐expression of Cosmc and the consequent restoration of T‐synthase in LS174TTn(+) cell were confirmed by Western blotting. B, The expression of Tn antigen in LS174TTn(+) cells transfected with wide‐type Cosmc was detected by FACS. C, Reduced H‐Ras expression was detected by western blotting in the transfected Tn positive cells together with the increased expression of E‐cad and reduced expression of N‐cadherin, Snail and Vimentin in protein level. D, Decreased mRNA expression of H‐Ras and corresponding changes of reversed EMT were analysed by Real‐time PCR in the LS174TTn(+) cells transfected with COSMC compared with the control cells (*P < 0.05 and **P < 0.01)