Figure 2.

Neural induction of porcine induced pluripotent stem cells (iPSCs) using dual SMADi with different seeding cell densities. A, Scheme of neural progenitor cells (NPCs) derivation using dual SMAD inhibitors (dSMADi), SB431541 (TGF‐b inhibitor) and LDN 193189 (BMP4 inhibitor) to promote neural induction. B, Gene expression analysis of NPCs from porcine iPSCs using RT‐PCR. The experiment was replicated three times. C, mRNA expression levels (Mean ± SEM) of SOX2, p75 and POU5F1 in dual SMAD‐inhibited NPCs derived from iPSCs analysed by quantitative real‐time PCR. Within the same target mRNA, values with different superscript letters are significantly different (P < 0.05). The experiment was replicated three times