Figure 4.

Combined treatment of FGF8, retinoic acid (RA) and SHH for patterning of iPSC‐NPCs. Comparative real‐time PCR expression of (A) HOXB4, (B) GFAP, (C) MBP and (D) Tuj1 in differentiated cells. The expression (Mean ± SEM) was measured by comparative real‐time PCR relative to the expression in non‐patterning control group. Samples were normalized to the housekeeping gene RN18S. The experiments were performed in biological triplicates. Within the same target mRNA, values with different superscript letters (a‐c) within each column indicate significant differences between groups (P < 0.05)