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. 2019 Jan 4;23(3):2093–2102. doi: 10.1111/jcmm.14118

Figure 5.

Figure 5

Neuropeptide W‐23 (NPW‐23)‐enhanced differentiation of ATDC5 cells through the protein kinase A, protein kinase C, ERK1/2, and p38 signalling pathways. Cells were cultured in differentiation medium (1% ITS) and incubated with or without NPW‐23 (200 ng/mL). On day 7 of the culture process, the cells were in the presence or absence of specific inhibitors of the different signalling pathways (CYM50769; PD98059; SB203580; Chele; H‐89). After 12 h, the total RNA was extracted and (A) Coll II, (B) aggrecan, and (C) p21 mRNA were detected by quantitative RT‐PCR. Each experiment was repeated at least three times. The data represent triplicate samples in two independent experiments. *P < 0.05 vs control group, # P < 0.05 vs NPW‐23 group.