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. 2019 Feb 11;10:42. doi: 10.3389/fgene.2019.00042

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Copyright © 2019 Zhang, Li, Sun, Li, Li, Jiang, Li, Liu, Han, Li, Wang, Tian, Kang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induction of differentiation in intramuscular and abdominal preadipocyte. The basic medium consisted of DMEM/F-12 and 10% FBS. The induction differentiation medium consisted of basic medium, insulin, dexamethasone, 3-isobutyl-1-methylxanthine, and oleate. The maintenance medium consisted of basic medium, insulin, and oleate. The induction differentiation medium was replaced with the maintenance medium at 48 h, whereas the maintenance medium was replaced with basic medium at 96 h.