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. 2019 Feb 11;10:42. doi: 10.3389/fgene.2019.00042

FIGURE 6.

FIGURE 6

lncRNA IMFNCR functions as a miRNA sponge. (A) IMFNCR knockdown decreased PPARG and FABP4 expression. (B) IMFNCR knockdown inhibited intramuscular adipogenesis, as indicated by Oil Red O staining and quantified by microplate reader at 510 nm. (C) IMFNCR knockdown inhibited the protein expression of PPARG. (D) IMFNCR knockdown increased the expression of miR-128-3p and miR-27b-3p. (E) miR-128-3p and miR-27b-3p inhibited the expression of IMFNCR. (F) miR-128-3p and miRNA mixture overexpression inhibited Rluc expression of the Rluc activity of psiCH2-IMFNCR-WT, while psiCH2-IMFNCR-WT-Mut no longer responded to miRNA mixture (miR-128-3p and miR-27b-3p). MiR-128-3p and miR-27b-3p mimics were transfected into DF1 cells, together with psiCH2-IMFNCR-WT, or psiCH2-IMFNCR-WT-Mut. The data represent means ± SEM (n = 3). ∗p ≤ 0.05; ∗∗p ≤ 0.01.