Figure 3.

Transcriptions of tight junction proteins claudin‐1 and occludin in the ileum and the colon. Claudin‐1 and occludin mRNAs in the terminal ileum and the transversal colon were normalized to β‐actin and cyclophilin A and their relative transcriptions are depicted. The preterm germ‐free (GF), Lactobacillus rhamnosus GG‐colonized (LGG), Salmonella Typhimurium‐infected (ST) and L. rhamnosus GG‐colonized and S. Typhimurium‐infected (LGG+ST) piglets were compared. One‐way analysis of variance (anova) with Tukey’s multiple comparison post‐hoc test was used to compare the groups and the values are presented as individual dots and mean (horizontal line). Statistical differences P < 0·05 are marked with different letters above the dots and the same letter indicates no significant differences. Six samples in each group were analyzed.