Skip to main content
. 2019 Feb 15;15:1744806919830018. doi: 10.1177/1744806919830018

Figure 2.

Figure 2.

TLR4 deficiency causes reduction of TRPV1 expression in DRG and alleviation of visceral hypersensitivity in TNBS-induced colitis. (a) TLR4 and TRPV1 transcription in DRG of WT and TLR4-deficient group post TNBS treatment. (b and c) TLR4 and TRPV1 protein level in DRG of WT and TLR4-deficient mice post TNBS treatment. Differences in the expression level at different time points within group were analyzed using repeated measures one-way ANOVA followed by Bonferroni’s multiple comparisons test (*p < 0.05; **p < 0.01; ***p < 0.001). Differences in expression level at a given time points between two groups were compared with student’s t test (#p < 0.05; ##p < 0.01; ###p < 0.001). (d) TLR4 deficiency resulted in significant alleviation of visceral hypersensitivity in TNBS-induced colitis mice compared to control mice (*p < 0.05; **p < 0.01; ***p < 0.001; two-way ANOVA followed by Bonferroni post hoc test). WT: wild type; TLR4: Toll-like receptor 4; KO: knockout; TNBS: 2,4,6-trinitrobenzene sulfate; TRPV1: transient receptor potential vanilloid subtype 1; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; mRNA: messenger RNA.