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. 2019 Feb 13;15:1744806919828921. doi: 10.1177/1744806919828921

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Flow cytometric analysis of intracellular nociceptin and membrane NOP in blood leukocyte subsets. MFI represents the amount of intracellular nociceptin or membrane NOP in blood leukocytes. Whole blood was cultured with or without PMA 10 ng/ml for 24 h. Nociceptin and NOP protein levels in leukocyte subsets in blood samples without PMA co-incubation served as controls. Data are presented as changes in nociceptin or NOP protein levels in subsets in the PMA group relative to the respective controls. Medians and interquartile ranges, n=12 for each group. *P <0.05, **P <0.01, ***P <0.005, ****P <0.0001. PMA: phorbol-12-myristate-13-acetate; NOP: nociceptin opioid peptide receptor.

Inline graphic — Flow cytometric analysis of intracellular nociceptin and membrane NOP in blood leukocyte subsets. MFI represents the amount of intracellular nociceptin or membrane NOP in blood leukocytes. Whole blood was cultured with or without PMA 10 ng/ml for 24 h. Nociceptin and NOP protein levels in leukocyte subsets in blood samples without PMA co-incubation served as controls. Data are presented as changes in nociceptin or NOP protein levels in subsets in the PMA group relative to the respective controls. Medians and interquartile ranges, n=12 for each group. *P <0.05, **P <0.01, ***P <0.005, ****P <0.0001. PMA: phorbol-12-myristate-13-acetate; NOP: nociceptin opioid peptide receptor.