Table 1.
Two gene clusters that showed the greatest transcriptional responses to co‐culture.
| Gene | Log2 fold change | Annotation/likely function |
|---|---|---|
| 1. Clustered genes concerned with sulfur metabolism and siroheme synthesis in Ruminococcus bromii, downregulated when in co‐culture with Blautia hydrogenotrophica | ||
| L2‐63_01134 | −2.82 | sbp Sulfate starvation induced protein |
| L2‐63_01135 | −3.01 | cysW_1 Sulfate transport system permease protein |
| L2‐63_01136 | −3.29 | cysW_2 Sulfate transport system permease protein |
| L2‐63_01137 | −3.12 | cysA Sulfate/thiosulfate import ATP‐binding protein |
| L2‐63_01140 | −1.81 | hemA Glutamyl‐tRNA reductase |
| L2‐63_01141 | −1.7 | cysG_1 Siroheme synthase, precorrin‐2 dehydrogenase |
| L2‐63_01142 | −1.73 | hemC Porphobilinogen deaminase |
| L2‐63_01143 | −1.77 | cysG_2 Siroheme synthase, uroporphyrin‐III‐C‐methyltransferase |
| L2‐63_01144 | −1.89 | hemB Delta‐aminolevulinic acid dehydratase |
| L2‐63_01145 | −1.39 | hemL2 glutamate‐1‐semialdehyde aminotransferase |
| L2‐63_01146 | −2.65 | iscR_1 HTH‐type transcriptional regulator |
| L2‐63_01150 | −2.85 | trxA_3 Thioredoxin‐M |
| L2‐63_01151 | −3.05 | bifunctional sulfur carrier protein |
| L2‐63_01152 | −2.68 | moeZ probable adenyltraferase/sulfurtransferase |
| L2‐63_01153 | −2.67 | mec CysO‐cysteine peptidase |
| L2‐63_01154 | −2.35 | trxB_2 Thioredoxin reductase, alkyl hydroperoxide reductase subunit |
| L2‐63_01155 | −2.09 | ifcA fumarate reductase subunit precursor |
| L2‐63_01156 | −2.11 | Ferredoxin II, NADH‐plastoquinoine oxidoreductase subunit |
| L2‐63_01157 | −2.13 | cysD Sulfate adenylyl transferase subunit 2 |
| L2‐63_01158 | −2.52 | cysN Sulfate adenylyl transferase subunit 1 |
| 2. Putative branched chain amino acid fermentation gene cluster in Blautia hydrogenotrophica, upregulated when in co‐culture with Ruminococcus bromii | ||
| RUMHYD_00546 | 8.27 | ilvE branched chain amino acid transaminase |
| RUMHYD_00547 | 7.66 | branched chain amino acid transport protein |
| RUMHYD_00548 | 6.48 | brnQ branched chain amino acid transport system II carrier protein |
| RUMHYD_00549 | 8.54 | 2‐oxoacid:acceptor oxidoreductase gamma subunit |
| RUMHYD_00550 | 9.28 | thiamine pyrophosphate enzyme |
| RUMHYD_00551 | 9.34 | pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP binding |
| RUMHYD_00552 | 7.27 | 4Fe‐4S binding domain protein |
| RUMHYD_00553 | 8.74 | hypothetical protein |
| RUMHYD_00554 | 9.42 | buk butyrate kinase (presumed acyl kinase) |
| RUMHYD_00555 | 9.46 | ptb phosphate butyryltransferase (presumed phosphate acyltransferase) |
| RUMHYD_00556 | 1.4 | transcriptional regulator, Gnt R family |
Transcripts correspond to those within the circles (1) and (2) in Fig. 4 C and D.