Figure 4.

PDS5B Is a Target of miR-223

(A) Four public algorithms—miRanda, miRDB, PicTar, and TargetScan—were used to predict that PDS5B might be a potential target of miR-223. (B) Real-time RT-PCR assay was performed to detect the mRNA level of PDS5B in PC cells treated with miR-223 mimics. EV, empty vector; miR-223, miR-223 mimics. ***p < 0.001 versus control. (C) Top: western blotting analysis was used to measure protein level of PDS5B in PC cells treated with miR-223 mimics or inhibitor. Bottom: quantitative results are shown for the top panel. EV, empty vector; Inhibitor, miR-223 inhibitor. ***p < 0.001 versus control. (D) Sequences of wild-type and mutant target sites for miR-223 in PDS5B are shown. (E) Luciferase reporter assays were performed in 293T cells to identify the binding of miR-223 to PDS5B 3′ UTR. wt, wild-type; mt, mutation. ***p < 0.001 versus control.