Skip to main content
. 2019 Jan 29;14:583–592. doi: 10.1016/j.omtn.2019.01.009

Figure 5.

Figure 5

Overexpression of PDS5B Reverses miR-223-Mediated Cell Growth Promotion and Apoptosis Inhibition

(A) Left: Real-time RT-PCR was used to measure the mRNA level of PDS5B in 293T cells after PDS5B siRNA transfection. Right: real-time RT-PCR was performed to measure PDS5B mRNA level in PC cells after PDS5B cDNA transfection. NC, nonspecific control siRNA; siPDS5B, PDS5B siRNA; EV, empty vector; PDS5B, PDS5B cDNA transfection. (B) MTT assay was used to measure the cell growth in PC cells after PDS5B overexpression plus miR-223 overexpression. Control, empty vector and nonspecific miRNA; miR-223, miR-223 mimics; PDS5B, PDS5B cDNA; Both, miR-223 mimics plus PDS5B cDNA transfection. **p < 0.01; ***p < 0.001 versus control; ##p < 0.01 versus miR-223 overexpression or PDS5B overexpression alone. (C and D) Apoptosis was measured in Patu-8988 (C) and Panc-1 (D) cells after PDS5B downregulation plus miR-223 inhibitor treatment. Control, nonspecific miRNA plus control siRNA; siPDS5B, PDS5B siRNA; Both, PDS5B siRNA plus miR-223 inhibitor. **p < 0.01; ***p < 0.001 versus control; #p < 0.05 versus miR-223 inhibitor or PDS5B siRNA transfection alone. (E) Western blotting was used in PC cells after PDS5B downregulation plus miR-223 inhibitor treatment. Control, nonspecific miRNA plus control siRNA; 223 inh, miR-223 inhibitor; siPDS5B, PDS5B siRNA; Both, PDS5B siRNA plus miR-223 inhibitor.