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(A) Activity-based proteasome profiling using probe 97 at different concentrations. Cocktail ABPs were added as control. (B) Left: competitive ABPP assay using ABP 97 and the inhibitors 1 (LU-102, 0.1 μM), 4 (LU-002c, 0.3 μM), and 5 (LU-002i, 3 μM). Right: competitive ABPP assay with probe 97 side-by-side with the three-probe mixture used previously in competitive ABPP experiments and the inhibitors 1 (LU-102, 0.1 μM), 4 (LU-002c, 0.03 μM), and 5 (LU-002i, 3 μM).
