Figure 12.

Preparation for correlative light and electron microscopy (CLEM). The flow chart illustrates the general schemes for the preparation of various specimens for flat embedding. The diagram includes all common specimen types from single cells to tissue sections, adherent cells, several immobilization procedures, and preparation of specimens which cannot be immobilized in principle (e.g. HPF frozen living cells), conservative fixation and embedding of fragile objects with a filter system and pre- and post-embedding CLEM. The possibilities of analytical investigations with the corresponding preparation or instrumental conditions are indicated.