Table 2.
Affinity-based proteomics results comparing aPL+ SLE (n = 66) vs SSA/SSB+ SLE (n = 63) subgroup. The proteins with the lowest p values comparing the two suggested subgroups are shown
| Gene | Uniprot ID | Protein name | Protein function1 | p value 2aPL+ vs SSA/SSB+ SLE | Fold change3 SSA/SSB+ vs. aPL+ SLE |
|---|---|---|---|---|---|
| ITGB1 | P05556 | Integrin beta-1 | Cell adhesion, host-virus interaction, integrin | 1.90E−10 | 3.4 |
| SLC13A3 | Q8WWT9 | Solute carrier family 13 member 3 | Ion transport, sodium transport | 2.20E−08 | 2.3 |
| CERS5 | Q8N5B7 | Ceramide synthase 5 | Lipid biosynthesis/metabolism, sphingolipid metabolism | 2.70E−08 | 2.7 |
| MSX2 | P35548 | Homeobox protein MSX-2 | Osteogenesis, transcription, transcription regulation | 2.80E−08 | 1.5 |
| F3 | P13726 | Tissue factor. Coagulation factor III | Blood coagulation, hemostasis | 3.60E−08 | 1.7 |
| HSP90AA1 | P07900 | Heat shock protein HSP 90-alpha | Stress response, chaperon | 5.00E−08 | 2.6 |
| MMP8 | P22894 | Neutrophil collagenase. Metalloproteinase 8 | Collagen degradation | 5.40E−08 | 2.1 |
| CTSB | P07858 | Cathepsin B | Thiol protease | 6.10E−08 | 1.3 |
| MMP10 | P09238 | Stromelysin-2 | Collagen degradation | 1.30E−07 | 1.9 |
| YARS | P54577 | Tyrosine--tRNA ligase | Protein biosynthesis, aminoacyl-tRNA synthetase | 5.30E−07 | 2.1 |
| SELE | P16581 | E-selectin | Cell adhesion | 7.70E−07 | 2.4 |
| FMO1 | Q01740 | Dimethylaniline monooxygenase [N-oxide-forming] 1 | Catalyzes the N-oxygenation of secondary and tertiary amines | 9.90E−07 | 1.8 |
| SAMD8 | Q96LT4 | Sphingomyelin synthase-related protein 1 | Lipid metabolism, sphingolipid metabolism | 3.40E−06 | 2.1 |
| ETNPPL | Q8TBG4 | Ethanolamine-phosphate phospho-lyase | Aminotransferase, lyase, transferase | 5.70E−06 | 1.3 |
| ARID2 | Q68CP9 | AT-rich interactive domain-containing protein 2. BAF200 | Transcription, transcription regulation, chromatin regulator | 8.90E−06 | 1.9 |
| ETV7 | Q9Y603 | Transcription factor ETV7 | Repressor | 1.20E−05 | 1.4 |
| CD40 | P25942 | Tumor necrosis factor receptor superfamily member 5. B-cell surface antigen CD40 | Receptor for TNFSF5/CD40LG | 1.50E−05 | 1.6 |
| KRT7 | P08729 | Keratin. type II cytoskeletal 7 | Viral process, blocks interferon-dependent interphase and stimulates DNA synthesis in cells | 4.10E−05 | 0.8 |
| TYK2 | P29597 | Non-receptor tyrosine-protein kinase TYK2 | Kinase, transferase, tyrosine-protein kinase | 6.70E−05 | 1.4 |
| REN | P00797 | Renin. Angiotensinogenase | Aspartyl protease, hydrolase, protease | 7.30E−05 | 1.4 |
| APCS | P02743 | Serum amyloid P-component | DNA-binding and opsonizing protein | 9.10E−05 | 0.9 |
| CYR61 | O00622 | Insulin-like growth factor-binding protein 10 | Growth factor binding, heparin-binding | 0.00013 | 0.9 |
| CLDN16 | Q9Y5I7 | Claudin 16 | Ion transport, transport | 0.00014 | 1.7 |
| GOT1 | P17174 | Aspartate aminotransferase | Aminotransferase, transferase | 0.00017 | 1.3 |
| EGF | P01133 | Pro-epidermal growth factor | Growth factor | 0.00027 | 1.2 |
1Protein function/biological process according to www.uniprot.org [54]
2p value obtained by Wilcoxon rank-sum test and Bonferroni correction for multiple testing
3Fold change is reported comparing the two subgroups. Values above 1 means upregulated in the SSA/SSB+ subgroup and values below 1 that the protein was downregulated in the SSA/SSB+ subgroup. Mean fold change of more than 100% is highlighted in italics