Fig. 6.

SOX17 overexpression suppresses DNA repair and damage response genes though transcriptional regulation. a KYSE510 radio-resistant cells (KYSE510-R-EV and KYSE510-R-SOX17) were treated with cisplatin 1 μM, radiation 2 Gy, or CCRT treatments. After 24 h, cells were harvested and analyzed for mRNA expression level by qRT-PCR. mRNA expression levels of DNA repair genes (upper) DNA damage response genes (lower) were inhibited in KYSE510-R-SOX17 cells in comparison with KYSE510-R-EV cells. b ChIP-qPCR showed that SOX17 bound to the promoter region of genes analyzed in KYSE510-R-SOX17 cells (black bars). ‘IgG’ used as a negative control (gray bars). c SOX17 downstream genes p21 and NFAT5 promoter activities were significantly inhibited in KYSE510-R-SOX17 compared to KYSE510-R-EV cells. Data represent mean ± S.D. from three independent experiments. P-values were determined by two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001