Fig. 8.

Overexpression of SOX17 suppresses DNA repair genes of xenograft derived from radiation resistant ESCC cell line to CCRT treatment. a Xenograft tissues were extracted for RNA and examined for mRNA expression of DNA repair genes and damage response genes. The RT-qPCR results showed that expression of BRCA1, DNAPK, p21, and RAD51 was inhibited in KYSE510-R-SOX17 cells in comparison with KYSE510-R-EV cells with (black bar) or without (black slashed bar) CCRT treatment. SOX17 mRNA levels are shown for comparison (right). P-values were determined by two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001. b Representative IHC results of BRCA1, p21, SIRT1 and SOX17 protein expression in four groups of xenografts. A 4-fold enlarged image of tumor area indicated by black box is shown in lower left inset for each xenograft (Original magnification: 100X)