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. 2019 Feb 18;7:24. doi: 10.1186/s40168-019-0632-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Positive correlation between MGEs co-occurring with ARGs and the total ARGs. Correlation of the log-transformed relative abundance of MGEs co-occurring with ARGs and the total ARGs from the gut of Piaratus mesopotamicus before, during, and after the antibiotic exposure. The black line indicates the regression model and the grey area correspond to the 95% confidence interval. Spearman’s correlation = 0.69, S = 1006, P = 9.4 × 10⁻⁰⁵. This result was corroborated using a bootstrap Spearman’s correlation (Spearman’s correlation = 0.69, bootstrap = 2000, bias = − 0.011, std. err = 0.14, Percentile interval 95% = 0.341–0.897)