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. 2019 Feb 18;12:35. doi: 10.1186/s13068-019-1374-2

Table 2.

Primers used in this study

Primers Sequences (5′–3′)a
Up-1 GTCTCTAGACATTGGCATTCTTCTATCACAAG (XbaI)
Up-2 GTCGTCGACTCCTCCTCCTGGCGGTGTTATT (SalI)
Short-1 GTCCTCGAGGGACTGGATCAGTCCTATG (XhoI)
Short-2 GTCCGGCCGTCCTCCTCCTGGCGGTGTTATT (EagI)
Down-1 GTCGCGCGCGGAGGAGGAGTAGACCCAGAAGAACCGGAG (MluI)
Down-2 GTCGGATCCACTACAACGCCTGCAGCATTC (BamHI)

aRestriction sites are underlined and indicated in following parentheses