Table 2.
Primers | Sequences (5′–3′)a |
---|---|
Up-1 | GTCTCTAGACATTGGCATTCTTCTATCACAAG (XbaI) |
Up-2 | GTCGTCGACTCCTCCTCCTGGCGGTGTTATT (SalI) |
Short-1 | GTCCTCGAGGGACTGGATCAGTCCTATG (XhoI) |
Short-2 | GTCCGGCCGTCCTCCTCCTGGCGGTGTTATT (EagI) |
Down-1 | GTCGCGCGCGGAGGAGGAGTAGACCCAGAAGAACCGGAG (MluI) |
Down-2 | GTCGGATCCACTACAACGCCTGCAGCATTC (BamHI) |
aRestriction sites are underlined and indicated in following parentheses