Crystal
structure of TET2-DNA complex.26 (a) TET2
binds dsDNA, breaks the double helix, and inserts 5mC into
its active site. (b) View of the TET2 active site binding 5mC by forming
hydrogen bonds with Asn1387, His1904, and Arg1261, all of which are
critical residues for TET2 catalytic activity and methylated DNA binding.
The oxidative iron center is shown in proximity to the methyl group
on 5mC. (c) A 2D rendering of the 5mC-bound TET2 active site and critical
residue interactions; blue = basic residue, red = acidic residue,
and black = neutral residue.