Fig. 3.

Mechanical and heat sensitivity in GHRHr KO mice at P21.

A-C: Male and female GHRHr+/− (Male: n = 9; Female: n = 17) and GHRHr−/− (Male: n = 5; Female: n = 5) mice show no differences in mechanical withdrawal threshold compared to WT controls (Male: n = 9; Female: n = 5) at P21 (Male: F_2,47, 1.2, p = 0.33; Female: F_2,24, 0.7, p = 0.5; Combined: F_2,47, 0.05, p = 0.96). D-E: Similar results are also found regarding heat withdrawal latencies in which male and female GHRHr mutants are no different than WT C57 mice (Male: H_9,9,5, 0.4, p = 0.83; Female: F_2,24, 1.6, p < 0.3). F: GHRHr+/− were found to be different than WT overall but this was not found to be different than GHRHr−/− mice (Combined: H_14,29,12, 6.6). # p < 0.04 vs WT but not GHRHr−/−. One-way ANOVA with Holm-Sidak post hoc test (parametric) or one-way ANOVA on Ranks’ with Dunn’s post hoc test (non-parametric) as appropriate.