Figure 1.

Effects of E₂β on H₂S production—role of CBS, CTH, and ER. (A) Primary uterine artery endothelial cells (UAEC) were treated with vehicle or estradiol-17β (E₂β, 10 nM) with or without the estrogen receptor (ER) antagonist ICI 182780 (ICI, 1 μM) for 48 h. Protein extracts (1 × 10⁶ cells) were used to determine hydrogen sulfide (H₂S) production. (B) UAEC were treated with vehicle or 10 nM E₂β for 48 h, and protein extracts were used to determine H₂S production in the presence or absence of an inhibitor of cystathionine β-synthase (CBS, cystathionine γ-lyase (CTH), or CHH and BCA, respectively. Data (means ± SEM) were collected from different cell preparations cells prepared from three to five ewes. *** P < 0.001 vs control.